rabbit anti tpi  (Proteintech)

Journal: Cell death & disease

Article Title: LDHA-mediated glycolysis in stria vascularis endothelial cells regulates macrophages function through CX3CL1-CX3CR1 pathway in noise-induced oxidative stress.

doi: 10.1038/s41419-025-07394-6

Figure Lengend Snippet: Fig. 2 Effects of oxidative stress on glycolysis pathway. A Schematic diagram illustrating the preparation of supernatant from SV-ECs following short-term stimulation of 500 μM H2O2 for 2 h. B Heat Map showing the differential expression analysis of SV-ECs treated with or without 500 μM H2O2. C–F SV-ECs were cultured in CCM with short-term stimulation of 500 μM H2O2 for 2 h and subsequently in SFM without H2O2 for another 24 h (C, D), or in SFM with long-term stimulation of 500 μM H2O2 for 48 h (E, F). Relative mRNA levels of glycolysis-related genes were analyzed by qRT-PCR (D, F). G, H Western blot analysis of the glycolysis-related genes in SV-ECs treated with H2O2 for 2, 24 and 48 h (n = 3). The data is expressed as Mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 analyzed by one-way ANOVA. ALDOA Fructose-bisphosphate aldolase A, CCM complete culture medium, ECs endothelial cells, G3P Glyceraldehyde-3-phosphate dehydrogenase, G6PI Glucose-6-Phosphate Isomerase, LC-MS/MS liquid chromatography tandem mass spectrometry; LDHA L-lactate dehydrogenase A chain, ns not significant, PGAM1 Phosphoglycerate mutase 1, PGK1 Phosphoglycerate kinase 1, PKM Pyruvate kinase, qRT-PCR Quantitative Reverse Transcription Polymerase Chain Reaction, SFM serum-free medium; SV stria vascularis, TPIS Triosephosphate isomerase.

Article Snippet: The membranes were then incubated overnight with the following primary antibodies as indicated: GPI (27978, 1:1000, SAB, Greenbelt, MD, USA), ALDOA (ab252953, 1:1000, Abcam), TPI1 (54087, 1:1000, SAB), G3P (1:1000, Abcam), PGAM1 (49976, 1:1000, SAB), PKM (7067T, 1:1000, CST, Danvers, MA, USA), LDHA (19987-1-AP, 1:1000, Abcam), PGK1 (ab199438, 1:1000, Abcam,) CX3CL1(29282, 1:500, SAB) and β-actin (20536-1-AP, 1:2000, Proteintech) overnight.

Techniques: Quantitative Proteomics, Cell Culture, Quantitative RT-PCR, Western Blot, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Reverse Transcription, Polymerase Chain Reaction

Journal: Cell death & disease

Article Title: LDHA-mediated glycolysis in stria vascularis endothelial cells regulates macrophages function through CX3CL1-CX3CR1 pathway in noise-induced oxidative stress.

doi: 10.1038/s41419-025-07394-6

Figure Lengend Snippet: Fig. 3 Effects of noise exposure on glycolysis pathway. A Schematic diagram illustrating the preparation of SV of cochleae after noise exposure. B Relative mRNA levels of glycolysis-related proteins in SV of C57BL/6 mice exposed to noise. C Western blot analysis of glycolysis pathway proteins in whole cochlear tissue homogenates of noise-exposed and control C57BL/6 mice. The data is presented as Mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 analyzed by one-way ANOVA. ECs endothelial cells, G3P Glyceraldehyde-3-phosphate dehydrogenase, G6PI Glucose-6-Phosphate Isomerase, LDHA L-lactate dehydrogenase A chain, ns not significant, PGAM1 Phosphoglycerate mutase 1, PGK1 Phosphoglycerate kinase 1, PKM Pyruvate kinase, qRT-PCR Quantitative Reverse Transcription Polymerase Chain Reaction, SV stria vascularis, TPIS Triosephosphate isomerase.

Article Snippet: The membranes were then incubated overnight with the following primary antibodies as indicated: GPI (27978, 1:1000, SAB, Greenbelt, MD, USA), ALDOA (ab252953, 1:1000, Abcam), TPI1 (54087, 1:1000, SAB), G3P (1:1000, Abcam), PGAM1 (49976, 1:1000, SAB), PKM (7067T, 1:1000, CST, Danvers, MA, USA), LDHA (19987-1-AP, 1:1000, Abcam), PGK1 (ab199438, 1:1000, Abcam,) CX3CL1(29282, 1:500, SAB) and β-actin (20536-1-AP, 1:2000, Proteintech) overnight.

Techniques: Western Blot, Control, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction